Novel Mass Spectrometric and Sampling Strategies for Investigating Neuropeptide Processing and Distribution
Kruse, Rebecca Anne
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https://hdl.handle.net/2142/84082
Description
Title
Novel Mass Spectrometric and Sampling Strategies for Investigating Neuropeptide Processing and Distribution
Author(s)
Kruse, Rebecca Anne
Issue Date
2002
Doctoral Committee Chair(s)
Sweedler, Jonathan V.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Neuroscience
Language
eng
Abstract
Direct mass spectrometric analysis, combined with knowledge of expressed genes, often provides enough information to identify peptides, to determine new processing pathways, and to assess genetic variations. In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.
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