Molecular Characterization and Utilization of the CAK1 Filamentous Viruslike Particle Derived From Clostridium Beijerinckii NCIMB 6444
Li, Yukun
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https://hdl.handle.net/2142/83742
Description
Title
Molecular Characterization and Utilization of the CAK1 Filamentous Viruslike Particle Derived From Clostridium Beijerinckii NCIMB 6444
Author(s)
Li, Yukun
Issue Date
2000
Doctoral Committee Chair(s)
Hans P. Blaschek
Department of Study
Food Science and Human Nutrition
Discipline
Food Science and Human Nutrition
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
The successful expression of the manA gene from Thermoanaerobacterium polysaccharolyticum in C. beijerinckii NCIMB 8052 and the sol operon of C. acetobutylicum DSM 792 in C. beijerinckii SA-2 when using pYL102E indicates that pYL102E can be used as an E. coli-C beijerinckii shuttle vector. This observation indicates the utility of CAK1-derived vectors for genetic engineering the solventogenic clostridia in order to improve the utilization of more complex substrates and for the production of specific end-products.
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