Genetic and Physiological Characterization of Clostridium Beijerinckii BA101 and NCIMB 8052
Chen, Chih-Kuang
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https://hdl.handle.net/2142/83738
Description
Title
Genetic and Physiological Characterization of Clostridium Beijerinckii BA101 and NCIMB 8052
Author(s)
Chen, Chih-Kuang
Issue Date
1999
Doctoral Committee Chair(s)
Hans P. Blaschek
Department of Study
Food Science and Human Nutrition
Discipline
Food Science and Human Nutrition
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
The addition of sodium acetate in MP2 medium was also able to stabilize solvent production by C. beijerinckii BA101, a hypersolvent producing mutant derived from NCIMB 8052. Moreover, BA101 demonstrated a greater increase in solvent production than NCIMB 8052 when acetate was added into MP2 medium. During the exponential growth phase, the specific activities of CoA transferase, acetoacetate decarboxylase and butyraldehyde dehydrogenase were higher in BA101 than in NCIMB 8052. mRNA expression levels of the sol operon were also elevated during the exponential and early stationary growth phases in BA101. The increased phosphotransbutyrylase activity, as a result of elevated ptb-buk operon expression, resulted in enhanced butyrate production by BA101 during the early exponential phase, which was offset by the elevated acid reassimilation due to an increase in CoA transferase activity. The results indicate that an increase in expression of the sol operon and, consequently, activities of associated enzymes may be, in part, responsible for enhanced solvent production by BA101 relative to NCIMB 8052.
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