Ozone Inactivation and Attachment of Listeria Monocytogenes to Abiotic Surfaces
Fisher, Christopher William
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https://hdl.handle.net/2142/83681
Description
Title
Ozone Inactivation and Attachment of Listeria Monocytogenes to Abiotic Surfaces
Author(s)
Fisher, Christopher William
Issue Date
2003
Doctoral Committee Chair(s)
Martin, Scott E.
Department of Study
Food Science and Human Nutrition
Discipline
Food Science and Human Nutrition
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Food Science and Technology
Language
eng
Abstract
L. monocytogenes 104035 and LO28 wild-type strains showed motility when gown at both 24 and 37°C although flagellin production was reduced during growth at 37°C. Both the wild-types and flagella minus mutants showed similar cells counts when attached over time on polystyrene microtiter plates and on stainless steel when gown at 24 and 37°C under static and shaking conditions. This suggests that flagella production in L. monocytogenes was not necessary for attachment and biofilm development. Scanning Electron Micrograph observations of L. monocytogenes gown on stainless steel chips for 24 hours and four days showed many cell aggregates formed on the surface, however no matrix of exopolymeric substances was seen.
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