Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
Lin, Li-Jung
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https://hdl.handle.net/2142/83627
Description
Title
Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
Author(s)
Lin, Li-Jung
Issue Date
2009
Doctoral Committee Chair(s)
Isaac Cann
Department of Study
Animal Sciences
Discipline
Animal Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
In our study, we characterized DinB and found that DinB was able to synthesize through abasic DNA and cyclobutane pyrimidine dimers, and these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of MacDinB was shown to facilitate interaction with the sliding clamp. Furthermore, individual mutations at different positions in the PIP-box led to identification of residues that are critical to PCNA/DinB interactions. Nevertheless, the biochemical analysis described here for DinB provides important insights into the properties, potential, and diversity of the Y-family of DNA polymerases.
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