Intestinal Inflammatory Responses to the Indigenous Microbiota
McCracken, Vance J.
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https://hdl.handle.net/2142/83533
Description
Title
Intestinal Inflammatory Responses to the Indigenous Microbiota
Author(s)
McCracken, Vance J.
Issue Date
2001
Doctoral Committee Chair(s)
Gaskins, H. Rex
Department of Study
Animal Sciences
Discipline
Animal Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Language
eng
Abstract
The potential for inflammatory cytokines to sensitize intestinal epithelial cells to indigenous bacteria was evaluated in HT-29 cells associated with Lactobacillus plantarum 299v in the presence or absence of tumor necrosis factor-alpha (TNF-alpha). Co-culture of TNF-alpha-stimulated HT-29 cells with L. plantarum 299v increased interleukin-8. (IL-8) mRNA expression above levels observed for cells treated with TNF-alpha alone. The ability of TNF-alpha-to upregulate CD14 and Toll-like receptor (TLR) expression and thereby enhance IL-8 mRNA expression in response to L. plantarum 299v was investigated. Baseline expression of CD14 mRNA and cell-surface CD14 was not altered by TNF-alpha. HT-29 cells did not express TLR2 mRNA. However TNF-alpha increased baseline HT-29 expression of TLR4 mRNA, and co-culture with L. plantarum 299v further enhanced TLR4 mRNA expression in TNF-alpha-treated HT-29 cells. These results indicate that neither CD 14 nor TLRs 2 and 4 are involved in the IL-8 mRNA response by HT-29 cells to L. plantarum 299v. However, these results indicate that intestinal epithelial cell responses to indigenous bacteria may contribute to intestinal inflammation.
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