Physiological Characterization of Acetotrophic Anaerobic Respiration Using Anaeromyxobacter Dehalogenans as a Model Organism
He, Qiang
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/83214
Description
Title
Physiological Characterization of Acetotrophic Anaerobic Respiration Using Anaeromyxobacter Dehalogenans as a Model Organism
Author(s)
He, Qiang
Issue Date
2003
Doctoral Committee Chair(s)
Sanford, Robert A.
Department of Study
Civl and Environmental Engineering
Discipline
Civl and Environmental Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Environmental Sciences
Language
eng
Abstract
Findings from this research identified the exceptional dechlorination ability of A. dehalogenans (116 mumol Cl- ·mg protein-1·h-1 ), and led to the discovery of its potential to couple growth to the reduction of heavy metals (Fe(III) and U(VI)), indicating a possible benefit of using these organisms for environmental clean-up. The ability of these organisms to successfully compete for nanomolar level acetate as the electron donor, in contrast to micromolar level required by methanogens, suggests that A. dehalogenans will successfully compete in environmental systems. The inability to use sub-nanomolar level acetate in chlororespiration, however, is attributed to the requirement for auxiliary energy to alleviate the toxicity of chlorophenols. Furthermore, the distinctive ability of A. dehalogenans to oxidize acetate in anaerobic respiration with diverse electron acceptors was investigated using biochemical and molecular approaches. Biochemical studies revealed characteristic components of acetotrophic respiration, such as the involvement of a c-type cytochrome. Representational difference analysis of cDNA further identified novel genes differentially expressed under chlororespiring conditions which will facilitate the identification of the specific proteins involved. Finally, the ecological importance of these organisms continues to grow as indicated by our and others' isolation of closely related organisms from different environmental samples. With a broad global distribution, A. dehalogenans serves as an ideal candidate for studying both biostimulation and bioaugmentation remediation technologies. Findings of this research lay the groundwork for future studies of A. dehalogenans in the bioremediation of mixed contaminants and for obtaining a fundamental understanding of the underlying biological principles.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
