Yield and Quality of Pathogen-Free Horseradish (Armoracia Rusticana) Planting Stock
Uchanski, Mark E.
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https://hdl.handle.net/2142/83122
Description
Title
Yield and Quality of Pathogen-Free Horseradish (Armoracia Rusticana) Planting Stock
Author(s)
Uchanski, Mark E.
Issue Date
2007
Doctoral Committee Chair(s)
Skirvin, Robert M.
Department of Study
Natural Resrouces and Environmental Sciences
Discipline
Natural Resrouces and Environmental Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Agricultural
Language
eng
Abstract
We first demonstrated that TuMV and Verticillium can be removed from horseradish plants by meristem tip culture in vitro. We also developed a sensitive, streamlined PCR-based testing protocol for Verticillium and a reverse transcriptase (RT)-PCR-based technique for TuMV to assess the pathogen status of horseradish cultivars, even in symptomless tissues. In the field, PF horseradish plants were more vigorous and productive than plants propagated by sets in the traditional manner (control). PF planting material produced higher yields than the control in all fields (high and low disease pressure). In many cases, PF plants produced two to three times the yield of the control. Additionally, 90% of the PF plants remained symptom-free (marketable) after one season in a high disease pressure field. Finally, we outline a field-based production protocol for rapid and cost effective propagation of PF horseradish sets. Using PF plants combined with complimentary cultural modifications, growers can regain yields and quality of roots, even into a second season.
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