Structural Transitions in Self-Assembled Lipid Systems Driven by Induced Curvature: From Cell -Penetrating Peptides to Programmable Vesicles
Mishra, Abhijit
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https://hdl.handle.net/2142/82865
Description
Title
Structural Transitions in Self-Assembled Lipid Systems Driven by Induced Curvature: From Cell -Penetrating Peptides to Programmable Vesicles
Author(s)
Mishra, Abhijit
Issue Date
2010
Doctoral Committee Chair(s)
Gerard Wong
Department of Study
Materials Science and Engineering
Discipline
Materials Science and Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
We also examined the interactions of other arginine rich cell-penetrating peptides, including Antp and oligoarginine, with model cell membranes, and find that the transduction activity correlates with induction of negative Gaussian curvature. The negative Gaussian membrane curvature is broadly enabling and its induction can lower the free energy barriers for a range of different entry mechanisms, such as direct translocation as well as endocytotic pathways. Furthermore, we show that the TAT peptide interacts strongly with actin cytoskeleton, which enhances membrane deformation and cytoskeleton reorganization necessary for endocytotic processes. We propose a mechanism that explains how a relatively simple molecule, like the TAT peptide, facilitates direct entry and multiple endocytotic mechanisms.
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