In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems
Slimmer, Scott Collins
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Permalink
https://hdl.handle.net/2142/82850
Description
Title
In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems
Author(s)
Slimmer, Scott Collins
Issue Date
2009
Doctoral Committee Chair(s)
Gerard Wong
Department of Study
Materials Science and Engineering
Discipline
Materials Science and Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Bioinformatics
Language
eng
Abstract
This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.
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