DNA Hybridization and Ligation for Directed Colloidal Assembly
Shyr, Margaret
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https://hdl.handle.net/2142/82849
Description
Title
DNA Hybridization and Ligation for Directed Colloidal Assembly
Author(s)
Shyr, Margaret
Issue Date
2009
Doctoral Committee Chair(s)
Braun, Paul V.
Department of Study
Materials Science and Engineering
Discipline
Materials Science and Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Materials Science
Language
eng
Abstract
Another conventional method of colloidal assembly we pursued was sedimentation via slow centrifugation. The salt requirement for DNA hybridization and ligation made it necessary to use slow salt addition through a dialysis membrane to achieve well-ordered colloidal crystals. Well-ordered hexagonally close-packed polystyrene colloid monolayers could be assembled by sedimentation, hybridization, and ligation. Layer-by-layer assembly is also possible on the sedimented colloidal crystals. Since the drying and liquid-air interface effects experienced during vertical deposition are not a factor for sedimented colloidal crystals, we attempted to use templated substrates, created by imprint and holographic lithography, to assemble non-FCC colloidal crystals.
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