University of Illinois Urbana-Champaign

Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28

Orr, Brent Alan

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https://hdl.handle.net/2142/82515

Description

Title
Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
Author(s)
Orr, Brent Alan
Issue Date
2007
Doctoral Committee Chair(s)
Roy, Edward J.
Department of Study
Neuroscience
Discipline
Neuroscience
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Language
eng
Abstract
Finally, a novel method of measuring the thermal stability of engineered antibody fragments using yeast surface display and flow cytometry was produced. This method allowed for the rapid determination of relative temperature stability without the need to produce large quantities of protein. This method compared favorably to other methods relying on soluble proteins and will facilitate the engineering and characterization recombinant proteins in the future.
Graduation Semester
2007
Type of Resource
text
Permalink
http://hdl.handle.net/2142/82515

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Dissertations - Neuroscience