Pituitary Adenylate Cyclase -Activating Peptide Signaling in the Mouse Circadian Clock
Lindberg, Peder Todd
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/82506
Description
Title
Pituitary Adenylate Cyclase -Activating Peptide Signaling in the Mouse Circadian Clock
Author(s)
Lindberg, Peder Todd
Issue Date
2005
Doctoral Committee Chair(s)
Gillette, Martha U.
Department of Study
Neuroscience
Discipline
Neuroscience
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Neuroscience
Language
eng
Abstract
Results from a variety of sources suggest a role of pituitary adenylate cyclase-activating peptide (PACAP) in light/glutamate-induced phase resetting of the circadian clock, yet the particulars of this role remain controversial. Mice generated by Lee Eiden and colleagues that do not express PACAP were examined to test the hypothesis that PACAP plays a physiological role in light or glutamate-induced circadian clock resetting. Both behavioral measurement of wheel-running and electrophysiological measurements of spontaneous electrical firing rate rhythms show a selective alteration in late-night clock resetting in transgenic mice, compared with their PACAP-competent cousins. This alteration in the resetting profile of PACAP-null mice is manifest despite the absence of any measurable change in the endogenous period of circadian rhythms. The suprachiasmatic nucleus (SCN) of the hypothalamus is the mammalian circadian pacemaker, which can be studied in an isolated slice preparation. Measurement and replacement of glutamate-induced signaling components to the SCN slice indicate that a permissive contribution of PACAP affects glutamate-induced signaling components downstream of cGMP. Concentration-dependent PACAP modulation of glutamate-induced shifting was supported by PACAP/glutamate co-administration to the isolated SCN slice. Together these experiments indicate that PACAP plays a number of intersecting roles that modify light/glutamate-dependent circadian clock resetting. A model is proposed that describes the current understanding of these intersecting pathways.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
