University of Illinois Urbana-Champaign

Messenger Ribonucleoprotein (Mrnp) Complexes: Proteomic and Functional Analysis

Ling, Shuo-Chien

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Description

Title
Messenger Ribonucleoprotein (Mrnp) Complexes: Proteomic and Functional Analysis
Author(s)
Ling, Shuo-Chien
Issue Date
2005
Doctoral Committee Chair(s)
  • Greenough, William T.
  • Vladimir I. Gelfand
Department of Study
Neuroscience
Discipline
Neuroscience
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
We provided the evidence that both microtubules and actin filaments are required for the proper localization of FMRP-RNP particles. Furthermore, FMRP associates with both microtubule- and actin-based motors, kinesin-1 and myosin Va. In order to unambiguously identify the detailed molecular mechanisms for RNA transport, we developed an in vivo functional assay and went on to provide evidence that dFMRP and associated mRNAs are actively transported by molecular motors. We were able to not only identify the motors that are required for the movement of dFMRP-RNP, but also look at the roles of conventional adaptors for the respective motors. Interestingly, both opposite-polarity motors, kinesin-1 and cytoplasmic dynein, are required for dFMRP-RNP transport, implying a coordination model rather than tug-of-war model for motor activities. Furthermore, while KLC is dispensable, dynactin is required for dFMRP-RNP transport, indicating a novel attachment of kinesin-1 to dFMRP-RNP.
Graduation Semester
2005
Type of Resource
text
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http://hdl.handle.net/2142/82504

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