University of Illinois Urbana-Champaign

Mechanisms of Asymmetric Protein Localization in Drosophila Embryonic Neural Stem Cells

Peng, Chian-Yu

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https://hdl.handle.net/2142/82488

Description

Title
Mechanisms of Asymmetric Protein Localization in Drosophila Embryonic Neural Stem Cells
Author(s)
Peng, Chian-Yu
Issue Date
2001
Doctoral Committee Chair(s)
Chris Doe
Department of Study
Neuroscience
Discipline
Neuroscience
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Neuroscience
Language
eng
Abstract
By screening a collection of deficiency lines and testing candidate gene involved in cell polarity, we identified lethal giant larvae (lgl) and discs large (dlg) to play key roles in the basal targeting of Prospero and Miranda. In embryonic and larval neuroblasts of lgl and dlg mutants, all basal targeted proteins become uniformly localized but distributions of the apical proteins are unaffected. In neuroblasts, Lgl protein is uniformly distributed at the cell cortex, and its cortical attachment is dependent on Dlg's apically enriched localization. Pharmacological studies suggest Lgl mediated basal targeting occur via a myosin-based transport mechanism, which is supported by genetic studies that show loss of Myosin II suppresses Lgl phenotype in neuroblasts. Taken together, we conclude that Lgl may function to suppress Myosin II activity and allow/promote positive Myosin dependent asymmetric localization in neuroblasts.
Graduation Semester
2001
Type of Resource
text
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http://hdl.handle.net/2142/82488

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