Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications
Ang, Ee Lui
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https://hdl.handle.net/2142/82405
Description
Title
Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications
Author(s)
Ang, Ee Lui
Issue Date
2007
Doctoral Committee Chair(s)
Zhao, Huimin
Jeffrey P. Obbard
Department of Study
Chemical Engineering
Discipline
Chemical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Chemical
Language
eng
Abstract
Another round of saturation mutagenesis on active site residues with 1-K31 as parent followed by random mutagenesis yielded the mutant 3-R21. Whole cell activity of 3-R21 for AN, 24DMA and 2IPA were 27.7, 9.8, and 12 nmol/min/mg protein respectively. The activities of 3-R21 for AN, 24DMA and 2IPA were improved by 8.9, 98.0, and 2.0-fold respectively over its parent 1-K31. In particular, the activity of the final mutant 3-R21 was improved by 3.5-fold over the WT AtdA enzyme. Overall, mutant 3-R21 had three mutations---V205A, 1248L, and S404C.
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