Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles
Ramsey, Joshua
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Permalink
https://hdl.handle.net/2142/82392
Description
Title
Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles
Author(s)
Ramsey, Joshua
Issue Date
2006
Doctoral Committee Chair(s)
Pack, Daniel W.
Department of Study
Chemical Engineering
Discipline
Chemical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Chemical
Language
eng
Abstract
While the hybrid vectors have combined some of the beneficial traits of the polymer and viral components the efficiency of the vector is low, in comparison to conventional viral vectors. Based on the changes in gene expression over time and the effect of chloroquine, the limiting step of hybrid vector cell transduction appears to be endolysosomal escape of the complex. The polymer component fails to mediate escape as efficiently as the MLV envelope protein, likely resulting in the degradation of internalized hybrid vector complexes. Incorporation of polymers better suited for mediating escape from the endolysosomal network could likely improve the hybrid vector efficiency.
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