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https://hdl.handle.net/2142/82366
Description
Title
Engineering Biosurfaces for Cell Studies
Author(s)
Gunawan, Rico Christian
Issue Date
2004
Doctoral Committee Chair(s)
Leckband, Deborah E.
Department of Study
Chemical Engineering
Discipline
Chemical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Chemical
Language
eng
Abstract
Biospecificity and the non-specific adsorption of proteins are two major issues for the use of biomaterials in implants and tissue engineering. This thesis describes efforts to reduce non-specific adsorption of proteins onto biomaterials and to enhance the biological activity of a biomaterial surface. In the first study, protein and cell resistance of poly(ethylene glycol) (PEG) and oligo(ethylene glycol) (OEG)-terminated self-assembled monolayers (SAMs) were studied as a function of the chain length of the OEG unit. In general, PEG with high molecular weight is better than short OEG SAMs in reducing protein and cell adhesion. In the second study, the effort to tune the biological activity of a material was achieved by microfluidic tools. These devices were used to pattern the biomaterial surface with covalently-immobilized gradients of extracellular matrix (ECM) proteins, laminin and collagen I. In turn, these surfaces were capable of directing the cell cycle progression and the migration of epithelial cells. This latter method of precisely controlling the cell environment is simple and broadly portable to other cell lines and to other ECM proteins or soluble factors.
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