Technologies for and Electrophysiological Studies of Structured, Living, Neuronal Networks on Microelectrode Arrays
Chang, John Chi-Hung
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https://hdl.handle.net/2142/80785
Description
Title
Technologies for and Electrophysiological Studies of Structured, Living, Neuronal Networks on Microelectrode Arrays
Author(s)
Chang, John Chi-Hung
Issue Date
2002
Doctoral Committee Chair(s)
Wheeler, Bruce C.
Department of Study
Electrical Engineering
Discipline
Electrical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Neuroscience
Language
eng
Abstract
We also recorded neurons patterned by photoresist image transfer and found patterned neurons capable of firing action potentials. Their firing depended on neuronal communication as action potentials were suppressed by high magnesium media. In addition, we found that network activity depended on network morphology. Well-confined networks fired action potentials independent of cell density and varied minimally in activity level, while less-confined networks had both greater dependence and variation. We attributed this effect to the greater glia:neuron interaction as both glial and synaptic density were higher in patterned than in random cultures. This interaction may depend on cell density, as synaptic density was equivalent in both patterned and random cultures when cultures were initiated at a lower plating density, implying that neuronal growth was stunted when culture is sparse. Currently, we cannot distinguish the direct effects of patterning on synaptic efficacy from the indirect effects of glia because our experiments were not designed to distinguish them. Future experiments should be conducted to test the hypothesis that distributed connectivity weakens the contribution of each synapse to cellular depolarization.
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