Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Dong, Chen-Yuan
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https://hdl.handle.net/2142/80655
Description
Title
Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Author(s)
Dong, Chen-Yuan
Issue Date
1998
Doctoral Committee Chair(s)
Gratton, Enrico
Department of Study
Physics
Discipline
Physics
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Physics, Condensed Matter
Language
eng
Abstract
Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, extension of two-photon microscopy to time-resolved polarization imaging of probe molecules inside STO cells are shown. Depending on the type of probe molecules and the nature of their interaction with cellular constituents, different degrees of rotational correlation times are obtained.
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