Measurement of Internal Movements of the Escherichia Coli Ribosome Using Forster Resonance Energy Transfer and Microsecond, Continuous -Flow Turbulent Mixing in Micro-Fabricated Devices
Majumdar, Zigurts Krishna
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https://hdl.handle.net/2142/80528
Description
Title
Measurement of Internal Movements of the Escherichia Coli Ribosome Using Forster Resonance Energy Transfer and Microsecond, Continuous -Flow Turbulent Mixing in Micro-Fabricated Devices
Author(s)
Majumdar, Zigurts Krishna
Issue Date
2005
Doctoral Committee Chair(s)
Clegg, Robert M.
Department of Study
Physics
Discipline
Physics
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The second half of this thesis is independent of the above. We present the design, construction and implementation of micro-fabricated, continuous-flow, turbulent mixing devices that can mix two or three fluids to complete homogeneity on the molecular scale in the microsecond range. The prototypical designs are compact, portable, simple to fabricate and consume smaller sample volumes than current technology. We characterize the turbulent mixing process in microfluidic channels with fluorescence intensity and lifetime imaging and show that both the dependence of mixing times and pressure drop on the flow velocity and Reynolds number agree well with theoretical expectations for turbulent pipe flow. The novelties in this work are: the new methods of fabrication which enable production of three-dimensional geometries in glass with micro-fabrication methods, the implementation of turbulent mixing and measurement at microsecond timescales and showing the ability to generalize theoretical expectations for turbulence in pipes with micrometer scale dimensions.
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