Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action
Snyder, Gregory E.
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Permalink
https://hdl.handle.net/2142/80497
Description
Title
Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action
Author(s)
Snyder, Gregory E.
Issue Date
2003
Doctoral Committee Chair(s)
Selvin, Paul R.
Department of Study
Physics
Discipline
Physics
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
"The other protein I studied is myosin V which walks bipedally along actin filaments taking steps which displace the center of mass 37 nm per step. A controversy over the nature of this movement was settled earlier this year by other members of our laboratory when they showed that myosin V processes in a hand-over-hand manner, with each of the heads alternating in the leading and trailing positions. To do this, they developed a new technique which they called FIONA for Fluorescence Imaging with One Nanometer Accuracy which allowed them to localize individual molecules of myosin V to within 1.5 nut on the surface of a microscope cover slip. I have extended and applied FIONA to a mutant of myosin V with a molecule of the enhanced Green Fluorescent Protein (eGFP) fused to one head of the dieter. I demonstrated that eGFP is suitable to use with FIONA, confirm the hand-over-hand model of myosin procession, and argue that in aggregate, the data on myosin's steps from our lab implies that myosin V adopts an asymmetric, ""telemark skier,"" configuration between steps. (Abstract shortened by UMI.)."
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