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https://hdl.handle.net/2142/79577
Description
Title
Chip -Based Detection of Protein Cancer Markers
Author(s)
Goluch, Edgar D.
Issue Date
2007
Doctoral Committee Chair(s)
Chang Liu
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
A similar approach was then employed to directly detect protein targets. Instead of DNA, monoclonal antibodies were patterned in the gap. The detector was then exposed to the protein target and gold nanoparticles functionalized with polyclonal antibodies. The target protein was used to immobilize the nanoparticle probes and silver staining was once again employed to bridge the gap It was determined that this technique can be used to detect protein targets both in buffer and serum samples with a 100 pM sensitivity limit.
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