University of Illinois Urbana-Champaign

Metabolic Response of Cells Exposed to Magnetomotion of Superparamagnetic Iron Oxide Nanoparticles

Flavin, Matthew Thomas

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https://hdl.handle.net/2142/78986

Description

Title
Metabolic Response of Cells Exposed to Magnetomotion of Superparamagnetic Iron Oxide Nanoparticles
Author(s)
Flavin, Matthew Thomas
Contributor(s)
Boppart, Stephen
Issue Date
2015-05
Keyword(s)
  • cellular metabolism
  • therapeutics
  • FLIM
  • magnetic nanoparticles
  • magnetomotion
  • remote-control
Abstract
Magnetomotion of superparamagnetic iron oxide nanoparticles (SPIONs) has recently been studied as a means to provide contrast in image modalities such as ultrasound and optical coherence tomography (OCT). Based on the current knowledge of mechanotransduction within cells, magnetomotion might be capable of inducing a biological effect which could make this technique useful for therapeutic applications. This thesis presents an experimental framework for quantifying the effects of SPION magnetomotion on cellular metabolism as a result of these pathways. Cells were treated by administering SPIONs to subcultures and exposing them to an alternating magnetic field gradient which induced magnetomotion through translational and rotational forces. The metabolic change was assessed using fluorescence lifetime imaging microscopy (FLIM) of endogenous nicotinamide adenine dinucleotide (NADH) molecules whose signal is known to be an indicator of metabolic activity. Evidence in earlier experiments suggests that such treatment is capable of producing an increase in cellular metabolism.
Type of Resource
other
Language
en
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http://hdl.handle.net/2142/78986

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