University of Illinois Urbana-Champaign

Liposome Encapsulated Manganese Chloride as a Liver Specific Contrast Agent for Magnetic Resonance Imaging

Niesman, Michael Ross

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Description

Title
Liposome Encapsulated Manganese Chloride as a Liver Specific Contrast Agent for Magnetic Resonance Imaging
Author(s)
Niesman, Michael Ross
Issue Date
1988
Doctoral Committee Chair(s)
Magin, Richard
Department of Study
Chemistry and Chemical Engineering
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, General
  • Chemistry, Pharmaceutical
Language
eng
Abstract
A study was undertaken to examine the possibility of using a liposome encapsulated paramagnetic ion (Mn$\sp{2+}$) as a contrast agent for magnetic resonance imaging (MRI) of the liver. Previous work with liposomes had shown that liposomes were concentrated in the liver after intravenous injection. Uptake of the vesicles into tumors was shown to be negligible. This suggested the possibility that the differences in uptake of the Mn$\sp{2+}$ in liposomes between liver and tumor might provide a means of highlighting tumors using MRI. Electron spin resonance (ESR) and proton nuclear magnetic resonance (NMR) relaxation were used in experiments to study the blood clearance, liver uptake and vesicle break down. The results showed that the Mn$\sp{2+}$ must be released from the vesicle and transferred to some extent to hepatocytes in order to bring about effective relaxation enhancement in liver tissue. After study of several different liposome preparations, it was found that vesicles composed of phosphatidylcholine, phosphatidylserine and cholesterol brought about the most rapid reduction in liver relaxation times. This vesicle preparation was injected intravenously into Fisher 344 rats with implanted liver tumors. The vesicles caused a marked reduction in the relaxation times of liver tissue, but little change in the relaxation times of the tumor. Magnetic resonance images obtained at 0.5 Tesla showed a large increase in the signal intensity in the liver and little change in the tumor. The results of this investigation showed the effectiveness of encapsulated Mn$\sp{2+}$ as an MRI contrast agent for liver metastases.
Graduation Semester
1988
Type of Resource
text
Permalink
http://hdl.handle.net/2142/77687

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