Characterization Of The Retinal Chromophore In The Gas-phase Via Photoisomerization Action Spectroscopy

Catani, Katherine Jean

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https://hdl.handle.net/2142/50884 Copy

Description

Title

Characterization Of The Retinal Chromophore In The Gas-phase Via Photoisomerization Action Spectroscopy

Author(s)

Catani, Katherine Jean

Contributor(s)

• Bieske, Evan
• Adamson, Brian D.
• Coughlan, Neville J

Issue Date

2014-06-17

Keyword(s)

Mini-symposium: Beyond the Mass-to-Charge Ratio: Spectroscopic Probes of the Structures of Ions

Abstract

The wavelength dependence for the photoisomerization of the isolated retinal protonated n-butylamine Schiff base (RPSB) is explored in the gas phase using a new technique that combines laser spectroscopy and ion mobility spectrometry. The technique involves exposing electrosprayed ions to tunable laser radiation as they are introduced into the drift region of an ion mobility spectrometer. Ions that absorb laser radiation photoisomerize, resulting in a detectable change in their drift speed through N$_2$ buffer gas. Without laser irradiation, 4 peaks are observed in the arrival time distribution of RPSB. The most intense and slowest peak is assigned as the all-{\emph{trans}} isomer by comparison with the calculated collision cross sections. With laser radiation, there is a clear depletion of the all-{\emph{trans}} isomer peak between 440 to 660 nm, corresponding to the S$_1$$\leftarrow$S$_0$ transition, with a maximum effect at 615$\pm$5 nm. There is also evidence of photoisomerization below 450 nm associated with the onset of the S$_2$$\leftarrow$S$_0$ transition. The photoisomerization action spectrum of RPSB is expected to mimic its absorption spectrum and should prove useful for calibrating theoretical descriptions of the isolated chromophore.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

English

Permalink

http://hdl.handle.net/2142/50884

DOI

https://doi.org/10.15278/isms.2014.TG06

Copyright and License Information

Copyright 2014 by the authors. Licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/

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