Towards cloning of a novel SCN resistance locus using de novo genome assembly and fosmid screening
Fliege, Christina
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https://hdl.handle.net/2142/72836
Description
Title
Towards cloning of a novel SCN resistance locus using de novo genome assembly and fosmid screening
Author(s)
Fliege, Christina
Issue Date
2015-01-21
Director of Research (if dissertation) or Advisor (if thesis)
Hudson, Matthew E.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
soybean
soybean cyst nematode (SCN)
Abstract
Glycine soja, the wild annual ancestor of soybean, has great potential to be used as a
source of genetic diversity for the crop. A Glycine soja line, PI 468916, contains a
resistance QTL to soybean cyst nematode (SCN) Heterodera glycines, a devastating pest
of soybean. This resistance QTL has been fine mapped in PI 468916 to a distance of
147kb. Through the use of whole genome sequencing with de novo assembly we were
able to gain information about the region. In this thesis the assembly data was used along
with fosmid sequences from both Sanger sequencing and Illumina reads to gain insight
into sequence differences between the Williams 82 reference genome and PI 468916 to
identify the gene(s) responsible for this resistance. The alignment of PI 468916 sequence
against the reference genome corresponding to the resistance QTL and the candidate
genes present in this region, is presented. This novel resistance locus could be used to
introduce new resistance in the crop.
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