Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents
Vincil, Gretchen
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https://hdl.handle.net/2142/72771
Description
Title
Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents
Author(s)
Vincil, Gretchen
Issue Date
2015-01-21
Director of Research (if dissertation) or Advisor (if thesis)
Zimmerman, Steven C.
Doctoral Committee Chair(s)
Zimmerman, Steven C.
Committee Member(s)
Hull, Kami L.
Mitchell, Douglas A.
Murphy, Catherine J.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
photobleaching
photostability
fluorescein
anti-fading agents
hyper-branched polymers
Abstract
The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases.
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