Studies of Protein Folding on Membranes and in Crowded Environments and Bridging the Research-Teaching Gap in K-12 Science
Denos, Sharlene
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https://hdl.handle.net/2142/72405
Description
Title
Studies of Protein Folding on Membranes and in Crowded Environments and Bridging the Research-Teaching Gap in K-12 Science
Author(s)
Denos, Sharlene
Issue Date
2009
Doctoral Committee Chair(s)
Martin Gruebele
Department of Study
Center for Biophysics and Computational Biology
Discipline
Biophysics and Computational Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Education, Sciences
Biophysics, General
Abstract
This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.
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