Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine
Spring, Bryan Quilty
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Permalink
https://hdl.handle.net/2142/72398
Description
Title
Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine
Author(s)
Spring, Bryan Quilty
Issue Date
2008
Doctoral Committee Chair(s)
Clegg, Robert M.
Department of Study
Center for Biophysics and Computational Biology
Discipline
Biophysics and Computational Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Abstract
The dissertation is concerned with biophysical applications of Forster resonance energy transfer (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of fluorescence spectroscopy and microscopy (in particular, FRET and FLIM) to biology and medicine. Several separate research topics are presented, including: investigation of the structural properties of a metal-DNA complex (M-DNA); development and characterization of a genetically encoded biosensor for monitoring intracellular redox potentials; image analysis for FLIM; and, a microfluidic mixing device for accessing microsecond reaction kinetics.
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