Hybrid Micro/nanofluidic Sampling Schemes for Mass Spectrometry
Iannacone, Jamie Marie
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https://hdl.handle.net/2142/72243
Description
Title
Hybrid Micro/nanofluidic Sampling Schemes for Mass Spectrometry
Author(s)
Iannacone, Jamie Marie
Issue Date
2008
Doctoral Committee Chair(s)
Sweedler, Jonathan V.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Abstract
Novel analytical sampling techniques are investigated in an effort to advance the exploration of chemical reactions underlying life processes. The analysis of substances found in living organisms is challenging and includes the precise collection, preparation, and identification of complex biological samples. An appropriate sampling scheme simplifies the collection, as well as the pretreatment procedures in order to allow for more direct chemical analyses. Here, hybrid micro/nanofluidic manipulation schemes are explored and interfaced with mass spectrometry in the effort to study the distribution and dynamic release of neurotransmitters and neuropeptides from individual neurons.
Molecular gate devices are composed of spatially isolated microfluidic channels connected via nanocapillary array membranes. The hybrid micro/nanofluidic tools are fabricated with poly(dimethylsiloxane) or poly(methyl methacrylate) in the presence of a polycarbonate membrane. Chips composed of each polymer substrate are successfully interfaced to electrospray ionization mass spectrometry, as stable electrosprays and high-quality mass spectra are generated. Furthermore, the poly(dimethylsiloxane)-based microchip emitter is used for on-line sample treatment prior to detection.
Porous polymer monoliths offer efficient mass transfer and high permeability as the nanoporous backbone interconnects to form multiple microfluidic flow-through channels. More commonly used as chromatographic media, the monoliths are investigated for their potential use as novel sampling tools. Fabricated in a capillary holder, porous polymer monoliths are interfaced with both electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry. The direct collection and subsequent detection of complex biological samples is further realized with the analysis of Aplysia californica bag cell releasate using matrix-assisted laser desorption/ionization mass spectrometry.
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