University of Illinois Urbana-Champaign

Storage Proteins in Apple Trees (Woody Plant, Nitrogen, Senescence)

Yoo, Won-Hyung

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Description

Title
Storage Proteins in Apple Trees (Woody Plant, Nitrogen, Senescence)
Author(s)
Yoo, Won-Hyung
Issue Date
1985
Department of Study
Horticulture
Discipline
Horticulture
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Plant Physiology
Abstract
  • This study was undertaken to elucidate into the mode of accumulation and subsequent degradation of storage proteins in apple trees. Protein isolation, characterization, and proteolytic enzyme study were involved.
  • The prominent proteins present in shoot bark were the MWs 56, 38, 28, and 20 kD. These 4 proteins constituted a high proportion of the total protein in bark increasing from 46 to 70% of the total protein during senescence. The 4 major proteins appeared to serve a storage role and consisted of many heterogeneous polypeptides of different isoelectric points. Twenty one, thirteen, seven, and twenty one heterogeneous polypeptides were found in those 4 major proteins, respectively. Each of the 4 major proteins had similar amino acid composition in their hydrolysates with approximately 40% of the total protein in basic amino acids, asparagine, glutamine, and their amides. The highest proteolytic activity was found when the 20 kD protein were used as a substrate.
  • During regrowth, a slightly greater degradation of the four dominant proteins was observed. A preferential degradation of some bark proteins differing in electrical charges was also found. Nonetheless, the protein degradation was not limited to a few protein species.
Graduation Semester
1985
Type of Resource
text
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http://hdl.handle.net/2142/71780

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