University of Illinois Urbana-Champaign

Isozyme and RFLP Studies in the Genus Glycine Willd. Subgenus Glycine

Menancio, Desiree Ibanez

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Description

Title
Isozyme and RFLP Studies in the Genus Glycine Willd. Subgenus Glycine
Author(s)
Menancio, Desiree Ibanez
Issue Date
1987
Doctoral Committee Chair(s)
Hymowitz, Theodore
Department of Study
Agronomy
Discipline
Agronomy
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, Molecular
  • Biology, Botany
Abstract
  • The genus Glycine Willd. subgenus Glycine consists of 12 wild perennial species which are native to parts of Australia, South China and adjacent islands in the west and south Pacific. This thesis investigated the variation and biosystematic patterns in the subgenus Glycine utilizing isozyme and RFLP analyses. Three studies were conducted and their results are described below.
  • First, isozyme variation among 90 accessions representing nine diploid species was analyzed by UPGMA cluster analysis. Ten defined clusters were revealed which were in agreement with classification based on morphology and cytogenetic evidence. The isozyme clusters showed high levels of between accession variation and differentiation among the accessions used. The isozyme evidence provided a good fit to the previously suggested evolutionary lines of development in the subgenus Glycine.
  • Second, isozyme analysis of 67 accessions of Glycine tabacina (Labill.) Benth. differentiated between diploid and tetraploid cytotypes in the species. Among tetraploid accessions, the isozyme banding patterns observed supported the claim that G. tabacina is an allopolyploid complex.
  • Third, total DNA from callus tissues of 28 accessions representing seven wild Glycine species were compared using recombinant genomic DNA probes from soybean and corn. Two of the heterologous probes from soybean detected restriction fragment length polymorphisms among the species used. RFLP analysis supported the previous grouping of G. tabacina, G. latifolia and G. microphylla into B-genome species.
Graduation Semester
1987
Type of Resource
text
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http://hdl.handle.net/2142/71641

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