University of Illinois Urbana-Champaign

The Genetics of Soybean Seed Urease (Isozymes, Clycine Max, Transcription)

Kloth, Reiner Hans

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Description

Title
The Genetics of Soybean Seed Urease (Isozymes, Clycine Max, Transcription)
Author(s)
Kloth, Reiner Hans
Issue Date
1985
Department of Study
Agronomy
Discipline
Agronomy
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Genetics
Abstract
  • The objectives were (i) to study the inheritance of the soybean seed urease isozymes, (ii) to determine the cause of the isozyme polymorphism and (iii) to study the inheritance of a soybean seed urease null.
  • Reciprocal crosses between cultivars homozygous for the electrophoretic slow and fast forms of seed urease were made. The results indicate that the fast and slow variants are inherited as codominant alleles.
  • Before begining a study to determine the source of the polymorphism between the fast and slow forms of seed urease, a speedier means of purifying urease is necessary. Seed urease was adsorbed to chloramphenicol-caproate substituted agarose. The enzyme was eluted when the ionic strength of the solution was increased and the pH decreased. Preliminary experiments show a thousand-fold purification of urease with a low yield.
  • The soybean seed urease null phenotype was previously described as lacking seed urease activity and antigen. Null lines behave similarly when crossed to cultivars homozygous for the slow or fast alleles of seed urease: the F(,1) seed from crosses of urease expressing cultivars with nulls express the urease allele of the cultivar and a 3:1 segregation for the presence and absence of urease is observed in progeny from F(,1) and heterozygous F(,2) plants. Examination of F(,2) and F(,3) urease positive seed revealed that this seed from fast x null crosses are all phenotypically urease fast, while the same seed from slow x null crosses shows a segregation of one seed that contains a fast urease either exclusively or in a heterozygous state with the slow isozyme for every 69 phenotypic slows. The data indicates that the urease null is caused by a cis-acting, epistatic allele linked to, but distinct from the isozyme locus. The urease nulls all carry an unexpressed fast form of seed urease.
Graduation Semester
1985
Type of Resource
text
Permalink
http://hdl.handle.net/2142/71617

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Agronomy