The Effects of 5-Fluorouracil on The Secretory Process of The Rat Parotid Gland (Protein)
Sandborg, Rebecca Ruth
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Permalink
https://hdl.handle.net/2142/71443
Description
Title
The Effects of 5-Fluorouracil on The Secretory Process of The Rat Parotid Gland (Protein)
Author(s)
Sandborg, Rebecca Ruth
Issue Date
1986
Department of Study
Physiology and Biophysics
Discipline
Physiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Animal Physiology
Abstract
Experimental animals were injected intraperitoneally with 100 mg/kg 5-fluorouracil for three days. The total volume, amylase and protein content of cannulated parotid saliva were determined following stimulation with either 5 mg/kg pilocarpine or 5 mg/kg isoproterenol in experimental, pair-fed, and control animals. Saliva from experimental animals was significantly lower in volume, amylase and protein content than both control groups. Sodium dodecyl sulfate, anionic and cationic gel electrophoresis of parotid saliva revealed no qualitative alterations in the types of proteins secreted. 5-fluorouracil treatment reduced the total glandular amylase per unit DNA in both unstimulated and isoproterenol-stimulated parotid glands. Decreased protein synthesis may be the mechanism underlying depleted secretory protein stores since the contents of isolated secretory granules from experimental parotid glands contained less radiolabelled protein than either control group and whole gland homogenates showed marked reductions in the activities of three lysosomal enzymes and total RNA content. Experimental animals contained less labelled protein in their secretory granules than controls, but secreted a greater proportion of their total glandular radiolabelled secretory protein into saliva relative to amylase suggesting that newly synthesized secretory proteins are preferentially secreted.
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