Influence of Rhodococcus Equi on the Respiratory Burst of Resident Alveolar Macrophages From Horses
Brumbaugh, Gordon Wayne
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/71339
Description
Title
Influence of Rhodococcus Equi on the Respiratory Burst of Resident Alveolar Macrophages From Horses
Author(s)
Brumbaugh, Gordon Wayne
Issue Date
1986
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Veterinary Science
Health Sciences, Immunology
Abstract
Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. Opsonized Rhodococcus equi are phagocytosed by equine alveolar macrophages but are ineffectively killed. The respiratory burst of equine neutrophils is obtunded by R. equi. If the bacteria similarly influence equine alveolar macrophages, that could partially explain its facultative intracellular survival.
The purpose of this study was to evaluate the influence of R. equi on the superoxide anion production, measured spectrophotometrically as the reduction of cytochrome C, and hexose monophosphate shunt activity, measured by ('14)CO(,2) liberation from ('14)C-1-D-glucose, of alveolar macrophages from horses. Alveolar macrophages were harvested from 6 anesthetized, healthy, light-breed, adult horses by bronchoalveolar lavage. Following a randomized complete block design, the suspension of cells was divided into aliquots of 10('6) viable alveolar macrophages which were exposed to 1, 10, or 100 g. of opsonized R. equi or opsonized zymosan A at 37 C for 2 hours.
The amount of superoxide anion produced by alveolar macrophages stimulated with three masses each of opsonized zymosan A and opsonized R. equi was not significantly different from that produced by unstimulated cells. The hexose monophosphate shunt activity was enhanced by both stimulants in a mass-related manner. No significant difference was found in the response produced by equal masses of either stimulant.
In this study the respiratory burst of equine alveolar macrophages was only evidenced by the hexose monophosphate shunt activity and superoxide anion was not coincidently produced. Rhodococcus equi did not adversely affect that response. The insignificant superoxide anion production by the alveolar macrophages suggests that this may not be a significant oxygen metabolite in those cells.
Further studies directed at defining the production of superoxide anion by equine alveolar macrophages should be performed. Studies of bacterial interaction with alveolar macrophages from foals should also be performed.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
