Suppression of T-Helper Cell Function by a Soluble Immunosuppressive Factor From Plasmodium Berghei Infected Rat Erythrocytes
Srour, Edward Fayik
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/71332
Description
Title
Suppression of T-Helper Cell Function by a Soluble Immunosuppressive Factor From Plasmodium Berghei Infected Rat Erythrocytes
Author(s)
Srour, Edward Fayik
Issue Date
1986
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Abstract
A soluble factor derived from Plasmodium berghei infected rat or mouse erythrocytes, but not control erythrocytes, exhibited immunosuppressive properties when tested in vivo. Mice treated with the immunosuppressive factor (ISF) and then immunized with sheep red blood cells (SRBC) had a reduced number of plaque forming cells (PFC) as compared to mice injected with control preparations. The delayed type hypersensitivity reaction elicited by SRBC was also suppressed in mice treated with the ISF. Purification by affinity chromatography and analysis by SDS-PAGE revealed that the ISF had a molecular weight of approximately 30 KD. Neither nylon wool enriched nor unfractionated spleen cells of mice treated with the ISF contained cells capable of suppression the in vitro response to SRBC. Macrophages from treated mice restored the PFC response of macrophage-depleted spleen cells as efficiently as those from control mice. B-cells from treated and untreated mice responded similarly when supplemented with the appropriate T-helper cells. T-helper cells from ISF treated mice failed to supplement naive B cells in a primary in vitro anti-SRBC response as successfully as cells from untreated mice. Interleukin 2 production was not affected by the ISF. Treatment of infected mice with the ISF resulted in higher parasitemia levels and earlier mortality. On the contrary, mice immunized with the ISF then infected with P. berghei had lower levels of parasitemia and lived longer than control mice.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
