Immunogenicity and Quantitation of Soluble Anaplasma Marginale Antigens From Cell Cultures and Blood
Moteane, Mohlalefi
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/71304
Description
Title
Immunogenicity and Quantitation of Soluble Anaplasma Marginale Antigens From Cell Cultures and Blood
Author(s)
Moteane, Mohlalefi
Issue Date
1983
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Abstract
Short-term Anaplasma marginale cultures were initiated utilizing infected bovine blood from a splenectomized calf at various periods during acute parasitaemia employing Medium 199 supplemented with 10% adult bovine serum and 25 mM HEPES buffer. Better growth was observed in cultures initiated with lower percentage of parasitized erythrocytes (PPE) than those at higher PPE. Mineral supplementation (KCl and or MgSO(,4)) did not improve the ability of medium to support anaplasmal growth and appeared to be deleterious.
Immunization of cattle with selected supernatants administered along with an adjuvant resulted in production of specific antibody as measured by the indirect fluorescent antibody test. Some of the immunized cattle were partially protected from parasitaemia following challenge with virulent Anaplasma marginale but not from anaemia associated with the disease.
Soluble A marginale antigens from plasma of a splenectomized calf during acute infection, water and sonic energy lysates of infected bovine and ovine erythrocytes, hydrochloric acid ('HCl') precipitated antigens and those derived from culture supernatants demonstrated identity in immunodiffusion tests.
Specific antibodies against soluble 'HCl' precipitated antigen from a water lysate of A marginale infected bovine erythrocytes were detected from convalescent sera of immunized and nonimmunized animals challenged with a virulent strain of A marginale by an indirect enzyme linked-immunosorbent assay (ELISA).
An ELISA inhibition test was used for quantitation of soluble A marginale antigens derived from cultures and blood. The quantity of soluble antigen was expressed in arbitrary units, with one unit defined as the amount of antigen capable of producing 50% inhibition of positive reference serum diluted 1:320. Supernatants obtained early in culture of A marginale appeared to yield more soluble antigens than those obtained late. Similarly those from cultures initiated at lower PPE had more antigens as compared with those initiated at higher PPE.
Evidence is presented which suggests that A marginale soluble antigen prepared from a sonic lysate of infected erythrocytes is complexed with a normal erythrocyte component.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
