Analysis of T-2 Toxin in Feed and Animal Specimens
Ramaswamy, Venkatachalam
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https://hdl.handle.net/2142/71292
Description
Title
Analysis of T-2 Toxin in Feed and Animal Specimens
Author(s)
Ramaswamy, Venkatachalam
Issue Date
1982
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Animal Pathology
Abstract
A gas liquid chromatographic method was developed for the determination of T-2 toxin in feeds. The T-2 toxin was extracted from feeds with aqueous methanol. A zinc acetate and aluminum chloride solution was used to denature and precipitate proteinaceous compounds, plant pigments and other extraneous materials. The T-2 toxin was selectively partitioned into the benzene fraction, which was washed with 1% potassium hydroxide to remove acidic compounds. Other extraneous materials were removed by silica gel column chromatography using acetonitrile as an elutant. The acetonitrile was removed by evaporation and heptafluorobutyryl imidazole (HFBI) was added to form esters of the toxin. The esters were separated on a 2% OV-1, 6ft. glass column and quantitated with a Nickel-63 electron capture detector. The absolute recovery of the T-2 toxin spiked at concentrations between 0.5-7.0 ug/g was 100.62 + 10.24%. The proposed method could detect 0.1 ug/g in mixed feeds.
T-2 toxin was selectively partitioned from plasma and urine into the benzene fraction. Extraneous materials were removed by silica gel column chromatography. Heptafluorobutyryl imidazole (HFBI) was added to the toxin to form esters. The HFBI derived T-2 esters were separated on a 2% OV-1 gas chrom Q 6 ft. glass column and quantitated with a Nickel-63 electron capture detector. HT-2 toxin was used as an internal standard. Recoveries of T-2 toxin from plasma and urine fortified with 0.1-3.0 ug toxin/ml ranged from 89-98% and 91-99% respectively. The proposed method could detect 0.01 ug toxin/ml in plasma and urine.
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