Purification, Characterization, Elisa Optimization and Dosage Effects of Beet Western Yellows Virus
Hewings, Adrianna Delong Whitcomb
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Permalink
https://hdl.handle.net/2142/70589
Description
Title
Purification, Characterization, Elisa Optimization and Dosage Effects of Beet Western Yellows Virus
Author(s)
Hewings, Adrianna Delong Whitcomb
Issue Date
1983
Department of Study
Plant Pathology
Discipline
Plant Pathology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Plant Pathology
Abstract
Best Western Yellows (BWY) was described as a plant disease of viral etiology in 1960. The adjective 'western' was selected to distinguish BWY from beet yellows (BY), a previously described yellowing disease of sugarbeet. BWY virus (BWYV) is believed to be of greater economic importance than BY virus because it is more prevalent and more difficult to control. Two broad objectives were defined for this research: (1) characterization of BWYV using current physiochemical techniques and (2) the determination of the effect of different doses of virus on hosts of BWYV.
Comparative purification of two luteoviruses (an isolate of barley yellow dwarf virus (BYDV) and from BWYV) revealed that the titer of BYDV peaked sharply in roots 10-14 days after inoculation whereas BWYV yields were consistently high from shoots harvested from 10 to more than 20 days after inoculation. Purified BYDV and BWYV had A260/280 ratios of 1.89 and 1.65 respectively.
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