Isolation and Characterization of the Chloroperoxidase Gene From Caldariomyces Fumago
Nuell, Mark Jay
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https://hdl.handle.net/2142/70559
Description
Title
Isolation and Characterization of the Chloroperoxidase Gene From Caldariomyces Fumago
Author(s)
Nuell, Mark Jay
Issue Date
1986
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Abstract
The chloroperoxidase gene (cpo gene) from the filamentous fungus, Caldariomyces fumago, was isolated within a 16.3 kilobase DNA fragment in the vector LambdaEMBL3. The DNA sequence of the gene and its proximal flanking regions was determined and analyzed by using the programs available on the BIONET National Computer Resource for Molecular Biology. Such analysis provided the primary sequence of the protein and identified regions of DNA sequence which have structures related by symmetry operations. By analogy with the structure of sequences known to function in the control of gene expression, these regions may be those responsible for the observed expression properties of the cpo gene. The actual function of DNA sequences in the flanking regions remains to be determined. Comparison of the genomic and cDNA versions of the cpo gene sequence shows that the gene lacks introns and does not have the typical AATAAA polyadenylation signal. The start site of transcription was mapped by primer extension analysis and found conform to the general pattern of eukaryotic transcription initiation sites. A search of a portion of the BIONET databases for sequences similar to that of the cpo gene did not find any significant matches.
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