Applications of Electrocatalyzed Chemiluminescence of Luminol (Hydrogen Peroxide, Glucose, Electrogeneration, Flow Injection)
Van Dyke, David Allen
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/70335
Description
Title
Applications of Electrocatalyzed Chemiluminescence of Luminol (Hydrogen Peroxide, Glucose, Electrogeneration, Flow Injection)
Author(s)
Van Dyke, David Allen
Issue Date
1986
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Abstract
During the past several decades, the luminol chemiluminescence (CL) reaction has been successfully applied to the sensitive determination of a variety of analytes. The research reported here made use of electrochemical methods both to catalyze the luminol CL reaction and to generate reagents for the CL reaction in situ. Although few analytical applications of luminol electrocatalyzed chemiluminescence (ECL) have been previously reported, the use of an electrode offers several potential advantages over the conventional metal ion or metal complex catalysts; these include control of both the spatial and temporal point at which catalysis takes place, and elimination of the need to prepare and add catalyst solutions. The emphasis of this research was on developing electrocatalysis of the luminol CL reaction and electrogeneration of reagents as viable methods for analysis.
A transparently-faced thin-layer electrochemical flow cell was constructed to allow detection of luminol ECL. Use of this cell in conjunction with flow injection analysis techniques provided a sensitive method for the determination of hydrogen peroxide (H(,2)O(,2)); the detection limit was 0.1 (mu)M and the log-log working curve was linear up to 0.1 mM, in spite of an ECL background from oxygen.
By the use of an immobilized enzyme column, the luminol ECL technique was extended to the determination of glucose; the detection limit was nearly as low as that achieved for H(,2)O(,2). A study was also made of combining electrocatalysis of the luminol CL reaction with a microporous membrane flow cell for glucose determination.
Extremely sensitive determination of luminol was carried out in a dual-electrode ECL cell, with simultaneous electrogeneration of H(,2)O(,2) at the upstream electrode; the detection limit was 0.1 nM. Preliminary studies were also made of using ECL detection (with concurrent H(,2)O(,2) electrogeneration) for the determination of analytes labelled with luminol or a related compound.
It was shown that luminol ECL can be used as a sensitive analytical technique; this research should provide the foundation for future exploitation of its advantages for analysis.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
