Analytical Chemiluminescence in Microporous Membrane Flow Cells
Pilosof, David
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https://hdl.handle.net/2142/70204
Description
Title
Analytical Chemiluminescence in Microporous Membrane Flow Cells
Author(s)
Pilosof, David
Issue Date
1982
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Abstract
Microporous membrane chemiluminescence (CL) flow cells are presented as an advantageous method for chemical reagent and analyte mixing in continuous flow. The method adds simplicity, significant reagent economy and minimal sample contamination to the conventional advantages of analytical CL determinations.
The most significant application of the proposed method is in coupling enzymatic reactions that generate hydrogen peroxide to CL detection, in the same flow cell, in spite of the very different pH optima required by both processes. The analysis of glucose, using this approach, is studied in depth, and results obtained are compared to values determined by a routine assay method with good correlation. Other applications include analysis by use of other oxidase enzymes, determination of mixtures and determination of sugars that can be enzymatically converted to D-glucose.
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