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https://hdl.handle.net/2142/68238
Description
Title
Taxonomic Study of Pasteurella Anatipestifer
Author(s)
Bangun, Arab
Issue Date
1981
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Veterinary Science
Language
eng
Abstract
Due to its proteolytic and nonfermentative activity suggestions have been made to place Pasteurella anatipestifer (the causal agent of duck septicaemia) under the genus Moraxella. However, presently it is listed under genus Pasteurella as species incertae sedis.
In this study over 120 isolates of P. anatipestifer (mostly from White Pekin ducks) were compared biochemically and culturally with Pasteurella and Moraxella species. The pathogenic and septicemic nature of P. anatipestifer with restricted host range (only avian species) provided preliminary evidence of its biological difference from Moraxella species) provided preliminary evidence of its biological difference from Moraxella species which is usually a common inhabitant of the mucous membrane of mammalian species. Pasteurella anatipestifer was clearly separated from genus Pasteurella by its proteolytic, nonfermentative, negative nitrate reductase and strict aerobic property. The high resistance of P. anatipestifer to polymyxin B and kanamycin, its short survival time at 37 C and ability to grow at 45 C, further differentiated P. anatipestifer from both Moraxella and Pasteurella species.
Minor serological cross reactions between P. anatipestifer and some Pasteurella species were detected which were of little significance for taxonomic purposes. Lack of any serological cross reaction between P. anatipestifer and Moraxella species indicated their unrelatedness.
Independency of P. anatipestifer from both genera was strengthened by its low DNA base composition (32 to 34 mole %) as compared to 40 to 42% for the genus Pasteurella and Moraxella as well as its lack of significant DNA-DNA homology to reference species of both genera. Low to high homology was, however, detected among P. anatipestifer strains.
Gas chromatography fingerprints of methylated bacterial cellular fatty acids of P. anatipestifer revealed stable species characteristics regardless of host of origin which were different from both Pasteurella and Moraxella species.
Polyacrylamide gel electrophoresis (PAGE) profiles of bacterial soluble proteins and esterase enzymes revealed differences between P. anatipestifer and Pasteurella species.
The results of this study are adequate enough to exclude P. anatipestifer from either genus Pasteurella or Moraxella and a proposal for a new genus is justified.
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