Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis
Osorno, Miguel
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https://hdl.handle.net/2142/68237
Description
Title
Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis
Author(s)
Osorno, Miguel
Issue Date
1981
Department of Study
Veterinary Medical Science
Discipline
Veterinary Medical Science
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Veterinary Science
Language
eng
Abstract
Soluble cell culture derived antigens of Babesia bovis were studied for their biophysical and chemical properties. The fraction found to be antigenically active by in vitro test and immunogenically potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a relatively heat stable glycoprotein.
Inoculation of cattle with a mixture of soluble B. bovis antigen and saponin adjuvant resulted in a prompt production of blood serum antibodies detectable by an indirect fluorescent antibody (IFA) test. These antibodies persisted at detectable levels for 3 to 4 months. Re-vaccination at 7 months following first inoculation resulted in an anamnestic immune response indicating a recognition and persistence of immunologic memory to primary sensitization.
Thirty 2-year old Hereford cattle which were inoculated with saponin fortified soluble B. bovis antigen, and 20 non-vaccinated similar animals experienced a natural outbreak of babesiosis upon introduction from a tick-free region into a babesiosis endemic area. All animals of the control groups developed signs of the disease and 8 of them died. Among vaccinated animals there was febrile response, hematologic abnormalities and parasitemia, however, all animals remained grossly clinically free of signs and continued to make adequate weight progress.
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