Light Scattering Measurement of Phospholipid Microvesicle Membrane Fusion Kinetics
Mckown, John Wayne
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https://hdl.handle.net/2142/67596
Description
Title
Light Scattering Measurement of Phospholipid Microvesicle Membrane Fusion Kinetics
Author(s)
Mckown, John Wayne
Issue Date
1981
Department of Study
Physiology and Biophysics
Discipline
Biophysics
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
The moments of the time constant density function, as measured by quasi-elastic light scattering and Koppel's method of cumulants, are expressed in terms of moments of the size density function of single-walled hollow spherical scatterers. An equation governing the evolution of fusing particles is derived and solved for simple cases. A numerical treatment is discussed and applied to a phospholipid vesicle system which appears to evolve primarily by membrane fusion. Detailed kinetic characterization of the evolution by means of light scattering measurements appears to be practical.
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