The Role of Rna and Rna Polymerases in The Aging Process
Knaus, Raymond Joseph
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https://hdl.handle.net/2142/67455
Description
Title
The Role of Rna and Rna Polymerases in The Aging Process
Author(s)
Knaus, Raymond Joseph
Issue Date
1981
Department of Study
Plant Pathology
Discipline
Plant Pathology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Radioactive uracil incorporated into the total RNA of old Rhizoctonia solani cells was twofold less than that in young cells, but there was no change in RNA label following a chase with unlabeled uracil. As the concentration of labeled uracil in the medium increased, the amount of uracil label in the cell pool and RNA increased in young and old cells. The amount of label in the cell pool and RNA was always less in the old cells than in young cells.
DNA-dependent RNA polymerases I, II and III were isolated from R. solani. Partial characterization of the polymerases showed that they are similar to other eukaryotic polymerases in size, complexity, template requirements, magnesium, manganese and ammonium sulfate optima and sensitivity or resistance to (alpha)-amanitin. Polymerase III was present at low levels in mature and old cells and was not detected in young cells. The levels of polymerases I and II decreased 90% with age without change in their in vitro specific activity.
Poly(A)-RNA was isolated from young and old R. solani cells. The poly(A)-RNA ranged in size from smaller than 5S to 28S with the majority 6S or larger in both cell ages. Young cells contained 2-3 times more poly(A)-RNA compared to old cells. In the rabbit reticulocyte lysate translation system, seven protein classes ranging in molecular weight from 24,000 to 60,000 daltons were translated from the poly(A)-RNA of young cells. Three protein classes ranging in molecular weight from 16,000 to 48,000 daltons were translated from the poly(A)-RNA of old cells.
Non-poly(A)-RNA (17S-6S) was isolated and represented 6% of the young and 4% of the old cells total RNA. These RNAs did not act as mRNA in the rabbit reticulocyte lysate translation system.
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