A Study of the Inactivation in Vivo of the Luciferase in the Luminous Marine Bacterium Beneckea Harveyi
Reeve, Carole Anne
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https://hdl.handle.net/2142/67410
Description
Title
A Study of the Inactivation in Vivo of the Luciferase in the Luminous Marine Bacterium Beneckea Harveyi
Author(s)
Reeve, Carole Anne
Issue Date
1981
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
The inactivation in vivo of the luciferase in stationary phase cultures of the luminous marine bacterium Beneckea harveyi was studied in an effort to elucidate its mechanism. Through studies utilizing inhibitors of metabolic energy production, an inhibitor of protein synthesis, and a mutant in which the luciferase was inactivated at a much slower rate, and through studies of intracellular ATP levels under various conditions, a model for the modulation of luciferase inactivation by a signal metabolite or metabolites of as yet unknown chemical identity was developed, and degradation of the luciferase to its constituent amino acids was ruled out as the direct cause of the inactivation. In a preliminary immunochemical study of the inactivation process, no inactive anti-luciferase cross-reacting material could be detected at any point during the inactivation under any conditions, suggesting that inactivated luciferase is in a form which is unrecognizable by antibodies directed against the native enzyme.
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