The Effect of Estrogen on Regulatory Enzyme Levels and Albumin Gene Expression in Xenopus Laevis Hepatocytes
Philipp, Barbara Wallner
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/67406
Description
Title
The Effect of Estrogen on Regulatory Enzyme Levels and Albumin Gene Expression in Xenopus Laevis Hepatocytes
Author(s)
Philipp, Barbara Wallner
Issue Date
1980
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
The estrogen induced synthesis of vitellogenin in male Xenopus laevis is a unique system in which to study the molecular mechanism of hormone action on gene expression. Following administration of estradiol-17 to male Xenopus laevis a specific set of egg yolk proteins together with all components required for the membrane dependent synthesis of a secretory protein are coordinately induced with vitellogenin synthesis.
In an effort to identify genes that are coordinately controlled by estrogen, I quantitated the activity of 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoA reductase), the key regulatory enzyme in cholesterol synthesis, and acetyl-CoA carboxylase and fatty acid synthetase, regulatory enzymes in the fatty acid synthetic pathway, during estrogen induced vitellogenin synthesis. HMG-CoA reductase and acetyl-CoA carboxylase activity show an increase in parallel with the synthesis of their respective endproducts upon administration of estrogen, while fatty acid synthetase activity is unaffected by the hormone.
The effect of estrogen on the constitutive synthesis of Xenopus serum albumin was examined by hybridization analysis, using cloned albumin cDNA as a probe. This clone was constructed by insertion of an RNA:DNA hybrid into pBR322 and identified by hybridization-translation assay and hybridization to RNA immobilized on diazo-benzyloxymethyl paper. A restriction map was constructed to further characterize the cloned sequence. Hybridization studies using this cloned albumin sequence determined that albumin synthesis was unaffected by estrogen and by the massive increase in vitellogenin synthesis and secretion.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
