Features of the Initiation of Translation of Two Plant Viral Messenger-Rnas

Browning, Karen Shive

Permalink

https://hdl.handle.net/2142/67398 Copy

Description

Title

Features of the Initiation of Translation of Two Plant Viral Messenger-Rnas

Author(s)

Browning, Karen Shive

Issue Date

1980

Department of Study

Biochemistry

Discipline

Biochemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Chemistry, Biochemistry

Language

eng

Abstract

Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5'-terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) RNA to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat germ 80S ribosomes protect a "limit" region 19-52 nucleotides from the 5'-terminus. Wheat germ 40S ribosomes protect a "limit" region 10-47 nucleotides from the 5'-terminus. Characterization of the ribosome protected regions from ribonuclease attack establishes that wheat germ 40S and 80S ribosomes form initiation complexes with a linear conformation of STNV RNA lacking the proposed 5'-terminal loop and stem anticipated by Leung et al. {(1979) Biochemistry 18, 1361-1366}. The 5'-terminus of the small virion mRNA of the cowpea strain of tobacco mosaic virus (C(,c)-TMV) contains a m('7) (5')ppp(5')Gp... cap group. The 5'-terminal sequence of this RNA is m('7) G(5')ppp(5')GUAUUUGAUGAUGGCAUACUCGAUUCCGACUC^C(,C)AG(,C)...(' ). The codons following the consecutive AUGs match the N-terminal amino acid sequence of the coat protein of C(,c)-TMV. Wheat germ 80S ribosomes protect regions adjacent to the two consecutive AUGs from ribonuclease attack. Therefore one or both of the AUG sequences serve as sites for the initiation of translation of the small virion RNA of C(,c)-TMV.

Graduation Semester

1980

Type of Resource

text

Permalink

http://hdl.handle.net/2142/67398

Owning Collections